FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_86.3510000005d540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_86.3510000005d540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53631
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA1340.24985549402397866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1300.021.0149418
TCCTTTT303.8479874E-421.0149418
TGCATTT303.8479874E-421.0149417
GTGTTTT2200.021.0149418
GCCTTTT507.1751856E-821.0149418
GTCATTT303.8479874E-421.0149417
GGCATTT405.2211435E-621.0149417
ATCTTTT609.949872E-1021.0149418
GAAATTT303.8479874E-421.0149417
GCGATTT303.8479874E-421.0149417
ACGTTTT507.1751856E-821.0149418
TATGTTT250.003324705221.0149417
GGTCTTT303.8479874E-421.0149417
TCGGTTT507.1751856E-821.0149417
GAAGTTT303.8479874E-421.0149417
GTTCTGT609.949872E-1021.0149420
CTCTTTT250.003324705221.0149418
TTTTCTG303.8479874E-421.0149421
TGTTTTT4550.021.0149419
ATGGTTT250.003324705221.0149417
Produced by FastQC (version 0.11.2)