FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_85.3510000005d526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_85.3510000005d526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40210
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA950.23625963690624224No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC440.10942551604078588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT12600.021.02366320
TTTCTGT558.241841E-921.0236621
GTTCTGT303.8141044E-421.0236620
TTTTCTG250.003302418921.0236621
TAGTACC38050.020.9975154
CTAGTAC37100.020.9975153
CGACAGC303.8464117E-420.99751310
TTGTTTT1050.020.99751318
TGGTTTT850.020.99751318
GTGTTTT1300.020.99751318
GGGTTTT1400.020.99751318
AGCTTTT405.2049254E-620.99751318
TCTAGTA34500.020.9975132
CGATAGG303.8464117E-420.99751310
TTCGTTT250.00332640820.99751317
GCTAGTA1550.020.9975132
GCGATTT354.4665096E-520.99751317
ACGTTTT303.8464117E-420.99751318
TGTCTTT250.00332640820.99751317
GAGTGCG250.00332640820.99751311
Produced by FastQC (version 0.11.2)