FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_84.3510000005d50c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_84.3510000005d50c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58108
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA2090.35967508776760515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA250.003311691321.03275921
GATTTTT2750.021.01464519
AATTTTT1450.021.01464319
GTTCTGT800.021.01464320
GGTTTTT5200.021.01464319
CATTTTT1800.021.01464319
ACTTTTT1300.021.01464319
TATCTGT354.4825898E-521.01464319
GCTCTGT456.1319406E-721.01464319
TCTTTTT1850.021.01464319
GCTTCTG250.003328312721.01464319
ATTTTTT7400.021.01464320
TATACAC303.8537555E-421.0146420
TGTTCTG303.8537555E-421.0146419
GGTTCTG303.8537555E-421.0146419
CACTGTC601.0004442E-921.0146419
ATTTCTG303.8537555E-421.0146420
ACAGTGC6150.020.996568
TGGTTTT1050.020.9965618
ACAGTCC2200.020.996568
Produced by FastQC (version 0.11.2)