FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_83.3510000005d4e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_83.3510000005d4e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72189
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA3020.41834628544515096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT1100.021.0097118
GTCATTT303.872433E-421.0097117
TATATTT303.872433E-421.0097117
TGCGTTT303.872433E-421.0097117
GGCGTAT303.872433E-421.0097115
TTATTTT558.589268E-921.0097118
TTTTCTG456.1886567E-721.0097121
GGATTTT1700.021.0097118
CAATTTT456.1886567E-721.0097118
CGGATTT456.1886567E-721.0097117
GGTTTCT558.589268E-921.0097119
ACATTTT558.589268E-921.0097118
AACTTTT456.1886567E-721.0097118
CGGGTTT558.589268E-921.0097117
GCTGTTT456.1886567E-721.0097117
AGTGTTT303.872433E-421.0097117
CCTTTTT1700.021.0097119
TAATTTT900.021.0097118
TCGTTTT900.021.0097118
GAATTTT456.1886567E-721.0097118
Produced by FastQC (version 0.11.2)