FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_82.3510000005d4c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_82.3510000005d4c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74163
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC2890.38968218653506465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT456.1235187E-721.0344621
GTTCTGT250.00331869421.03445820
AAATTTT950.021.02025818
AATTTTT4050.021.02025819
ACGTTTT950.021.02025818
TCGTTTT950.021.02025818
GAATTTT1250.021.02025818
AGCTTTT1050.021.02025618
TTATTTT1550.021.02025618
CGCTTTT1400.021.02025618
CAATTTT751.8189894E-1221.02025618
CCGTTTT1000.021.02025618
TAGTTTT1100.021.02025618
AACTTTT651.200533E-1021.02025618
TGCTTTT751.8189894E-1221.02025618
GTCTTTT1550.021.02025618
ACCTTTT558.54925E-921.02025618
CTGTTTT1300.021.02025618
CGGTTTT2400.021.02025618
TCATTTT701.4551915E-1121.02025618
Produced by FastQC (version 0.11.2)