FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_80.3510000005d477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_80.3510000005d477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47645
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC1140.23926959806905237No Hit
GGATAGTTAGCCTGTCTCTTATACACA560.11753594291111344No Hit
GGATAGTTAGCCGGGGGGGGTTTTTTT480.10074509392381152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT1400.021.03368621
TTTTCTG354.4294735E-521.03368621
GTTTCTG609.749783E-1021.03368420
ATTCTGT405.1605693E-621.03368420
ATTTCTG651.1459633E-1021.03368420
TGGTTTT1800.021.01156818
TCCTTTT250.003322371321.01156818
AAATTTT456.092323E-721.01156818
GATATTT250.003322371321.01156817
AGGATTT250.003322371321.01156817
GAAGTTT250.003322371321.01156817
TTATTTT507.1446266E-821.01156818
GCGGTTT354.4656656E-521.01156817
GGATTTT1150.021.01156818
CAATTTT507.1446266E-821.01156818
TAGTTTT701.4551915E-1121.01156818
ACATTTT456.092323E-721.01156818
CGGGTTT250.003322371321.01156817
GGATAGT40450.021.0115681
AGAGTTT250.003322371321.01156817
Produced by FastQC (version 0.11.2)