FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_8.3510000005cb80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_8.3510000005cb80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC252.0No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC100.8No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC100.8No Hit
ACAGTGCTCATGGCCGCGGGTTTTTTT40.32No Hit
ACAGTGCTCATGGAGCTGTCTCTTATA30.24No Hit
ACAGTGCTCATGCTGTCTCTTCTACCC30.24No Hit
ACAGTGCTCATGGGCGGGGGTTTTTTT30.24No Hit
ACAGTGCTCATGCGCTGTCTCTTATAC30.24No Hit
ACAGTGCTCATGGTGGCCTGTCTCTTA30.24No Hit
ACAGTGCTCATGCCCTGTCTCTTATAC30.24No Hit
ACAGTGCTCATGGGGTAGCGTTTTTTT30.24No Hit
ACAGTGCTCATGGGTGCGTCTTTTTTT20.16No Hit
ACAGTGCTCATGCTGGACCTGTCTCTT20.16No Hit
ACAGTGCTCATGGATAACGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGGTCTGTCTCTT20.16No Hit
ACAGTGCTCATGGCGGGTCGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGTTGGTTTTTTT20.16No Hit
ACAGTGCTCATGGAGGGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGCGGTGTTTTTTT20.16No Hit
ACAGTGCTCATGCGTGGTGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGAGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGCGGCGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGGAGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGCATGTTTTTTT20.16No Hit
ACAGTGCTCATGTGGGGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGACGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGCTTGCTTTTTTT20.16No Hit
ACAGTGCTCATGTTGTGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGCTGTGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGCCTGTCTCTTA20.16No Hit
ACAGTGCTCATGGCGGGGGGCTGTCTC20.16No Hit
ACAGTGCTCATGGGAACGGATTTTTTT20.16No Hit
ACAGTGCTCATGTGCTGTAGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGAGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGTGTGTTTGTTTTTTT20.16No Hit
ACAGTGCTCATGACAGGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGGGCTGTCTCTT20.16No Hit
ACAGTGCTCATGCGGTGCCGTTTTTTT20.16No Hit
ACAGTGCTCATGGGAGGTGCTTTTTTT20.16No Hit
ACAGTGCTCATGGGGGGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC20.16No Hit
ACAGTGCTCATGTCGGCGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGGGTCTGTCTCTTAT20.16No Hit
ACAGTGCTCATGGTGGGGGGTTTTTTT20.16No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCAT1200.021.0000025
CTCATGT301.6740938E-421.0000027
GTGCTCA1200.021.0000024
TTTTTTT750.021.021
GTTTTTT351.44566875E-521.020
CTCATGG401.1890661E-621.07
CTCATGA351.44566875E-521.07
GCTCATG1250.021.06
ACAGTGC1100.020.9999981
GGTTTTT250.001853412620.99999819
AGTGCTC1100.020.9999983
CAGTGCT1100.020.9999982
CTTTTTT250.001853412620.99999820
Produced by FastQC (version 0.11.2)