FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_79.3510000005d45e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_79.3510000005d45e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46329
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA590.12735003993179217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCT303.8555692E-421.00000219
CAAGATT303.8555692E-421.00000210
TCCTTTT354.481371E-521.00000218
CAAGACC303.8555692E-421.00000210
CAATGGT354.481371E-521.00000210
CAATGGG609.931682E-1021.00000210
CAAGAAG354.481371E-521.00000210
CAATGCC303.8555692E-421.00000210
CAGTTTT609.931682E-1021.00000218
CAATGAT303.8555692E-421.00000210
CAATGAG303.8555692E-421.00000210
GTCATTT303.8555692E-421.00000217
GCAAGCC354.481371E-521.0000029
GCGATTT303.8555692E-421.00000217
GCAAGAC609.931682E-1021.0000029
GCAAGAA701.4551915E-1121.0000029
GGCGTTT354.481371E-521.00000217
CAACCTG354.481371E-521.00000210
CAACCCG303.8555692E-421.00000210
GTTCTGT701.4551915E-1121.00000220
Produced by FastQC (version 0.11.2)