FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_78.3510000005d434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_78.3510000005d434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32903
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC1540.4680424277421512No Hit
AGTGTGACGAACCTGTCTCTTCTACAC560.17019724645169135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG303.8227983E-421.00000211
AACGCGT303.8227983E-421.00000210
AACGTGA303.8227983E-421.00000210
GTGTTTT609.622454E-1021.00000218
AACCGGA303.8227983E-421.00000210
GCGACGA303.8227983E-421.0000024
AACGACC303.8227983E-421.00000210
AACTGGA303.8227983E-421.00000210
TGATTTT303.8227983E-421.00000218
CGCTTTT303.8227983E-421.00000218
GAACGTT303.8227983E-421.0000029
GTATTTT456.0066486E-721.00000218
GAACGCT609.622454E-1021.0000029
TGCGACG303.8227983E-421.0000023
GAACGAA456.0066486E-721.0000029
GTTTTTT9650.021.00000220
CCGTTTT609.622454E-1021.00000218
GGTTTCT303.8227983E-421.00000219
AACCCGG456.0066486E-721.00000210
TAGTTTT456.0066486E-721.00000218
Produced by FastQC (version 0.11.2)