FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_75.3510000005d3d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_75.3510000005d3d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38631
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC651.1459633E-1021.0000021
ACTGTTT303.8394926E-421.00000217
TTTGACC950.021.0000021
GGGTCTG405.1906827E-621.00000218
CCAACGA38450.021.0000026
CGATATT303.8394926E-421.00000210
TGCGTTT303.8394926E-421.00000217
ACGTTTT405.1906827E-621.00000218
AACGAGA2400.021.0000028
TGACCAA950.021.0000023
GGACTTT303.8394926E-421.00000217
AACGACT1600.021.0000028
AACGACA1200.021.0000028
CGCTTTT405.1906827E-621.00000218
TTCGACC31900.021.0000021
CGAAGGG303.8394926E-421.00000210
TAGTTTT950.021.00000218
CGAGGTG303.8394926E-421.00000210
CGAGGGT800.021.00000210
AACTTTT303.8394926E-421.00000218
Produced by FastQC (version 0.11.2)