FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_73.3510000005d392.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_73.3510000005d392.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31591
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC500.15827292583330696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT850.021.00000218
TTGTCGT354.4232205E-521.00000210
GTTGAGC455.990405E-721.0000029
GTTGAAG455.990405E-721.0000029
GTTGAAA405.1409224E-621.0000029
AGCTTTT405.1409224E-621.00000218
TTGCAAG354.4232205E-521.00000210
GGGTTCG1400.021.0000023
AAATTTT455.990405E-721.00000218
CTGGGTT1400.021.0000021
GGACTTT405.1409224E-621.00000217
TGTCTGT354.4232205E-521.00000219
GTTCTGT405.1409224E-621.00000220
TGATTTT455.990405E-721.00000218
GCGGTTT455.990405E-721.00000217
ATGGTTC354.4232205E-521.0000022
CATTTTT1600.021.00000219
GGTTCGT30850.021.0000024
GCAATTT354.4232205E-521.00000217
CTAGGTT354.4232205E-521.0000021
Produced by FastQC (version 0.11.2)