FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_72.3510000005d385.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_72.3510000005d385.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33956
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC2740.8069266109082343No Hit
GTGTAGTGTGGCTGTCTCTTATACACA2360.6950170809282601No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC1000.29449876310519496No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC960.2827188125809872No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC930.2738838496878313No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC930.2738838496878313No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC590.17375427023206502No Hit
GTGTAGTGTGGACCTGTCTCTTATACA570.1678642949699611No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC510.15019436918364942No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC490.14430439392154554No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT450.13252444339733774No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA450.13252444339733774No Hit
GTGTAGTGTGGATACTGTCTCTTATAC360.10601955471787018No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC360.10601955471787018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT751.8189894E-1221.00000218
GGAGAGA354.4360724E-521.00000210
AATTTTT800.021.00000219
AGTAGTG751.8189894E-1221.0000022
GGCGTTT354.4360724E-521.00000217
GTCGTGT1600.021.0000023
TTATTTT405.1601364E-621.00000218
AGGGTTT354.4360724E-521.00000217
GTTTCTG701.2732926E-1121.00000220
CGTAGTG751.8189894E-1221.0000022
TCGTGTG1600.021.0000024
TGGAGCT405.1601364E-621.0000029
TGGAGAT701.2732926E-1121.0000029
TGGAGAA354.4360724E-521.0000029
ATTGTTT354.4360724E-521.00000217
AGTGTGG31950.021.0000025
TTTAGTG405.1601364E-621.0000022
TGGACTT405.1601364E-621.0000029
TGGACTC405.1601364E-621.0000029
TGGACTA354.4360724E-521.0000029
Produced by FastQC (version 0.11.2)