FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_7.3510000005cb66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_7.3510000005cb66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13556
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC610.4499852463853644No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT160.11802891708468574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT950.021.00000218
TGCCGGG354.187124E-521.00000210
TGTTTTT950.021.00000219
ATGCGAT354.187124E-521.0000029
GGGGGTT354.187124E-521.00000216
CCTTTTT354.187124E-521.00000219
ATGCCGC354.187124E-521.0000029
GATTTTT950.021.00000219
GGGATTT354.187124E-521.00000217
ATGTTTT354.187124E-521.00000218
AATGCTG701.0913936E-1121.0000028
AATGCCA557.183189E-921.0000028
AATGCAT557.183189E-921.0000028
AATGCAA354.187124E-521.0000028
TGCGGGC354.187124E-521.00000210
CGCTGTC354.187124E-521.00000219
CTACAGC250.00321269221.01
ACAGCAA250.00321269221.03
CTTCAGC10400.021.01
CGTCAGC506.277696E-821.01
Produced by FastQC (version 0.11.2)