FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_69.3510000005d328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_69.3510000005d328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58500
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC1000.17094017094017094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAA751.8189894E-1221.0145442
CCTTGAA53250.021.0145442
ACATTGA751.8189894E-1221.0145441
ATTGAAC751.8189894E-1221.0145443
ACCTTGA53300.021.0145441
TTTGAAC1550.021.0145423
CAGTCTC250.003328677521.01454221
TTTCTGT1000.021.01454221
TTTTCTG405.2355754E-621.01454221
TTTTTCT250.003328677521.01454221
ACTTTGA1550.021.0145421
TCTTGAA2250.021.0145422
ACCTAGA250.003328677521.0145421
CTTGAAC55450.021.0145423
CTTTGAA1550.021.0145422
CCTAGAA250.003328677521.0145422
TTGTTTT900.020.99658218
CAGTTTT751.8189894E-1220.99658218
TGGTAGT456.184546E-720.99658210
CTGGGTG1650.020.9965829
Produced by FastQC (version 0.11.2)