FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_67.3510000005d2e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_67.3510000005d2e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44618
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTGGGGGCTGTCTCTTA540.12102738804966606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTTT303.8302277E-421.0179617
TCGGTTT303.8302277E-421.0179617
GCGGTTT303.8302277E-421.0179617
TAGCTTT303.8302277E-421.0179617
GTTTCTG303.8302277E-421.0179620
CGGATTT303.8302277E-421.0179617
GACATTT303.8302277E-421.0179617
GCTGTTT303.8302277E-421.0179617
TGCTTTT609.786163E-1021.0179618
GGGATTT303.8302277E-421.0179617
CTGTTTT609.786163E-1021.0179618
GGTATTT303.8302277E-421.0179617
GTAGTTT303.8302277E-421.0179617
CGTTTTT2450.021.0179619
GGGTTTT1650.021.01795818
GCCTTTT507.0962415E-821.01795818
CAGTTTT701.2732926E-1121.01795818
CCTATTT250.00331326521.01795817
AGCTTTT701.2732926E-1121.01795818
ACGTTTT651.1459633E-1021.01795818
Produced by FastQC (version 0.11.2)