FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_61.3510000005d21c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_61.3510000005d21c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41654
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTGGCTGTCTCTTATACA1620.38891823114226726No Hit
GACTCTATGGTCTGTCTCTTATACACA1500.3601094732798771No Hit
GACTCTATGGTGCCTGTCTCTTATACA970.2328707927209872No Hit
GACTCTATGGTGTCTGTCTCTTATACA880.2112642243241946No Hit
GACTCTATGGTGACTGTCTCTTATACA550.13204014020262161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGT250.003305158321.02283920
GTTCTGT303.818329E-421.02283720
ATTTCTG354.4299966E-521.02283720
GGTGAGG1000.020.99769
GCCTTTT507.140807E-820.997618
GGTGACT507.140807E-820.99769
GGTGACA507.140807E-820.99769
CAGTTTT558.383722E-920.997618
CTATATG2150.020.99763
GTGTCGG250.003328325720.997610
ATCTTTT250.003328325720.997618
AGCTTTT405.212234E-620.997618
GTGTTGG354.4713688E-520.997610
AATCTAT558.383722E-920.99762
TATATGG2150.020.99764
ACGTTTT558.383722E-920.997618
TGCGTGG250.003328325720.997611
AGGATTT354.4713688E-520.997617
GGCGTTT354.4713688E-520.997617
GTTCTTT303.8495366E-420.997617
Produced by FastQC (version 0.11.2)