FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_60.3510000005d1f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_60.3510000005d1f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52184
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC2200.42158516020236086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT1750.021.0191941
GAATCCT45350.021.0191941
GATTCCT1450.021.0191941
GAATACT1250.021.0191941
CGCGACG303.8658243E-420.99904310
CGCGACC354.4969966E-520.99904310
CGCTGGA601.0022632E-920.99904310
GCGGGGT303.8658243E-420.99904311
CGCTGCG303.8658243E-420.99904310
GGGTCTT303.8658243E-420.99904316
AATCCTT45400.020.9990432
ACGCCTA354.4969966E-520.9990439
ACGCTTT303.8658243E-420.9990439
GCCTTTT701.4551915E-1120.99904318
CAGTTTT800.020.99904318
AGCTTTT701.4551915E-1120.99904318
ACGCCGG1800.020.9990439
ACGCTGC1200.020.9990439
ACGCCCC405.2511095E-620.9990439
ACGCCCA303.8658243E-420.9990439
Produced by FastQC (version 0.11.2)