FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_59.3510000005d1e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_59.3510000005d1e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54752
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA700.12784921098772647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCTA651.1823431E-1021.0000029
CAGTATG651.1823431E-1021.0000029
CAGTACA651.1823431E-1021.0000029
AGTGGGG1300.021.00000210
TCAGTTG5200.021.0000028
TCAGTGA4950.021.0000028
TGCCTCA53400.021.0000024
ATTGCCT651.1823431E-1021.0000022
AGTTGGT651.1823431E-1021.00000210
CAGTGCG1300.021.0000029
TTGCCTC651.1823431E-1021.0000023
AATTGCC651.1823431E-1021.0000021
CGTTTTT2600.021.00000219
TTGTTTT1150.021.018
TGGTTTT950.021.018
CAGTCTT601.0040822E-921.09
AAGTACC800.021.01
CAGTTTG1450.021.09
CAGTTTA701.4551915E-1121.09
ATCTTTT250.003339388921.018
Produced by FastQC (version 0.11.2)