FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_55.3510000005d16d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_55.3510000005d16d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47251
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1330.2814755243275274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCC7850.021.0000027
TGCGTTA558.445568E-921.00000210
TGCGCGG405.231741E-621.00000210
TGCCGGG800.021.00000210
TTATTTT558.445568E-921.00000218
TGTTTTT3800.021.00000219
TGCGAGG405.231741E-621.00000210
TGCTGGG800.021.00000210
TGCTGCG405.231741E-621.00000210
ATGCGCG1100.021.0000029
ATGCGCA800.021.0000029
ATGCGAG1100.021.0000029
ACTTTTT950.021.00000219
TCAGCAA40500.021.0000023
ATGCTGC558.445568E-921.0000029
ATGCCCG558.445568E-921.0000029
ATGCTCT405.231741E-621.0000029
ATGCTCA405.231741E-621.0000029
ATGCTAG800.021.0000029
TCGTTTT405.231741E-621.00000218
Produced by FastQC (version 0.11.2)