FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_54.3510000005d144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_54.3510000005d144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29814
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA2490.8351781042463271No Hit
CTGATCGTAGCACCTGTCTCTTATACA750.25155966995371304No Hit
CTGATCGTAGCAGCTGTCTCTTATACA480.16099818877037633No Hit
CTGATCGTAGCAGGGGCTGTCTCTTAT370.12410277051049842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG24800.021.0336151
CTGCTCG950.021.0336131
CCGATCG609.313226E-1021.0336131
CGGATCG1650.021.0336111
CTTATCG651.09139364E-1021.0336111
CAGATCG557.9817255E-921.0336111
TGGTTTT506.966911E-820.99832318
CAGTTTT250.003305683820.99832318
AGCTTTT506.966911E-820.99832318
TGCGTTT250.003305683820.99832317
GCGATTT250.003305683820.99832317
AATTTTT950.020.99832319
ACGTTTT506.966911E-820.99832318
CACGTTT250.003305683820.99832317
GCAGGTA250.003305683820.99832310
TTATTTT506.966911E-820.99832318
GATGTTT250.003305683820.99832317
GCAGGCG506.966911E-820.99832310
GCAGGAA250.003305683820.99832310
TGATCGT24850.020.9983232
Produced by FastQC (version 0.11.2)