FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_53.3510000005d11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_53.3510000005d11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22834
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC870.38101077340807565No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA430.18831566961548568No Hit
GAGCTACGTCTCTGTCTCTTATACACA400.17517736708417272No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC360.15765963037575545No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT300.13138302531312954No Hit
GAGCTACGTCTAAGGGCTGTCTCTTAT260.11386528860471228No Hit
GAGCTACGTCTAGTCTGTCTCTTATAC230.1007269860733993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT751.8189894E-1221.00000218
TCTAGGT651.07320375E-1021.0000029
TCTAGGC651.07320375E-1021.0000029
TCTAGCT651.07320375E-1021.0000029
TCTAGAG751.8189894E-1221.0000029
AATTTTT1300.021.00000219
TGCTACG751.8189894E-1221.0000022
GTCTATG1300.021.0000028
GTCTAGA1300.021.0000028
GTCTACA751.8189894E-1221.0000028
AGTTTTT1500.021.00000219
GTGCTAC751.8189894E-1221.0000021
TGGTTTT405.037633E-621.018
GTGTTTT609.1677066E-1021.018
GCCTTTT455.8402657E-721.018
CAGTTTT506.780465E-821.018
GGCATTT250.003280563721.017
TCTAGTG701.2732926E-1121.09
TCTAGTC405.037633E-621.09
TCTAGTA506.780465E-821.09
Produced by FastQC (version 0.11.2)