FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_52.3510000005d0f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_52.3510000005d0f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12570
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC1210.9626093874303899No Hit
AGACGTCGATACCTGTCTCTTCTACAC500.3977724741447892No Hit
AGACGTCGATACACCTGTCTCTTATAC130.10342084327764518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT303.6430705E-421.00000218
TATACAC303.6430705E-421.00000221
ACGTTTT303.6430705E-421.00000218
GATACGC751.8189894E-1221.0000028
GATACCT900.021.0000028
GATACAT608.039933E-1021.0000028
TACGGGG455.416168E-721.00000210
TGTTTTT1200.021.00000219
CCGTCGA751.8189894E-1221.0000023
TTATACA303.6430705E-421.00000220
TACGTGG303.6430705E-421.00000210
ATACGCA303.6430705E-421.0000029
GAATTTT303.6430705E-421.00000218
GCGTTTT303.6430705E-421.00000218
ATGTTTT455.416168E-721.00000218
GGGGTTT303.6430705E-421.00000217
TACCTGT455.416168E-721.00000210
GCCGTCG751.8189894E-1221.0000022
ATACTGT303.6430705E-421.0000029
ATACTGG303.6430705E-421.0000029
Produced by FastQC (version 0.11.2)