FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_51.3510000005d0d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_51.3510000005d0d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23821
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC3011.2635909491625037No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC1110.4659753998572688No Hit
GAGGAACAGAGCTGTCTCTTATACACA930.390411821502036No Hit
GAGGAACAGAGTGGGGGCTGTCTCTTA270.11334536753284917No Hit
GAGGAACAGAGTGGCTGTCTCTTATAC260.10914739095755845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT455.862803E-721.00000218
GAGTGTT303.7798274E-421.0000029
GAGTGGT1200.021.0000029
GAGGAAC20750.021.0000021
GAGTGCT455.862803E-721.0000029
GAGTGAT455.862803E-721.0000029
AGTGGTG609.222276E-1021.00000210
AGTGGGC303.7798274E-421.00000210
AGTGGCG303.7798274E-421.00000210
AGTGGAG303.7798274E-421.00000210
ATGAACA557.923518E-921.0000022
TAGTTTT303.7798274E-421.00000218
GATGAAC557.923518E-921.0000021
GAGTCGT303.7798274E-421.0000029
GAGTCGA303.7798274E-421.0000029
GAGTTGT303.7798274E-421.0000029
GAGTTGG900.021.0000029
GAGTTGC303.7798274E-421.0000029
GAGTTCG557.923518E-921.0000029
GAGTCAG303.7798274E-421.0000029
Produced by FastQC (version 0.11.2)