FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_5.3510000005cb16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_5.3510000005cb16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16846
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC750.4452095452926511No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC190.11278641814080492No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT180.10685029087023626No Hit
GAGCTACGTCTCTGTCTCTTATACACA170.10091416359966758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTG608.6583896E-1021.0000029
TCTAGTA303.7157867E-421.0000029
AAATTTT303.7157867E-421.00000218
CTCTTTT303.7157867E-421.00000218
GGATTTT303.7157867E-421.00000218
TCTACGG608.6583896E-1021.0000029
TCTATCT303.7157867E-421.0000029
TCTATAG303.7157867E-421.0000029
TCTAATT303.7157867E-421.0000029
TCTAATC303.7157867E-421.0000029
TGCTTTT303.7157867E-421.00000218
GGCTTTT303.7157867E-421.00000218
AGGTTTT303.7157867E-421.00000218
GTCTACA608.6583896E-1021.0000028
GCATTTT303.7157867E-421.00000218
ATTTTTT4650.021.00000220
CGATTTT303.7157867E-421.00000218
GTGTTTT455.6515273E-721.018
GGGTTTT354.2660395E-521.018
TCTAGTT455.6515273E-721.09
Produced by FastQC (version 0.11.2)