FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_49.3510000005d0a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_49.3510000005d0a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65412
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT1670.2553048370329603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG354.5180008E-521.00000211
ATGGGAG354.5180008E-521.00000211
CATGAAT354.5180008E-521.00000210
CATTGAG354.5180008E-521.00000210
CATTGAA354.5180008E-521.00000210
GATATTT354.5180008E-521.00000217
CCAGTTT354.5180008E-521.00000217
ATTAGGG354.5180008E-521.00000211
TTATTTT850.021.00000218
CATCTGG354.5180008E-521.00000210
GGATTTT1400.021.00000218
GAGCTTT354.5180008E-521.00000217
CATTCAC354.5180008E-521.00000210
CATCAGG354.5180008E-521.00000210
TCTGCTC60600.021.0000024
GGGGGTT354.5180008E-521.00000216
TCGCTTT354.5180008E-521.00000217
TGCTCCT354.5180008E-521.0000026
TCATGTA1400.021.0000029
CATAGTG354.5180008E-521.00000210
Produced by FastQC (version 0.11.2)