FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_48.3510000005d088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_48.3510000005d088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10991
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC1261.1463925029569648No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC430.3912291875170594No Hit
TAAGCGTGGAACTGTCTCTTATACACA150.13647529797106722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT658.731149E-1121.00000218
AGCGTGG10100.021.0000023
GGAACGC658.731149E-1121.0000028
GGAACCG556.7993824E-921.0000028
GGAACCA556.7993824E-921.0000028
TTGTTTT354.0918458E-521.018
TGGTTTT404.650581E-621.018
AATTTTT950.021.019
AACTGTA303.6015644E-421.010
GAGTGGA303.6015644E-421.04
TAAGCGT9900.021.01
AAGAGTG303.6015644E-421.02
GAACGTG303.6015644E-421.09
GAACGGG709.094947E-1221.09
AGTGGAA303.6015644E-421.05
GAACGAG303.6015644E-421.09
GGTTTTT1600.021.019
CATTTTT303.6015644E-421.019
TAAGAGT303.6015644E-421.01
GCGTGGA10600.021.04
Produced by FastQC (version 0.11.2)