FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_47.3510000005d06e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_47.3510000005d06e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16319
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC410.25124088485814083No Hit
TAGCAGACTGGACCTGTCTCTTATACA240.14706783503891172No Hit
TAGCAGACTGGAGCCTGTCTCTTATAC200.12255652919909309No Hit
TAGCAGACTGGAGCTGTCTCTTATACA180.11030087627918377No Hit
TAGCAGACTGGCTGTCTCTTATACACA170.10417304981922913No Hit
TAGCAGACTGGAAGCTGTCTCTTATAC170.10417304981922913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT354.25588E-521.00000218
GGATGGT250.003240793521.00000210
GTGTTTT250.003240793521.00000218
GGATGCC250.003240793521.00000210
AAATTTT250.003240793521.00000218
ACGTTTT250.003240793521.00000218
TGTTTTT701.0913936E-1121.00000219
TGGAGTG354.25588E-521.0000029
TGGAGTA250.003240793521.0000029
TGGAGGT354.25588E-521.0000029
TGGAGGG701.0913936E-1121.0000029
TGGAGGA506.482696E-821.0000029
TGGAGCT354.25588E-521.0000029
TGGAGCG354.25588E-521.0000029
TGGAGCA250.003240793521.0000029
GAGTTTT250.003240793521.00000218
TGGAGAT354.25588E-521.0000029
CTGGATC1000.021.0000028
TGGACTG250.003240793521.0000029
TGGATTG250.003240793521.0000029
Produced by FastQC (version 0.11.2)