FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_46.3510000005d045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_46.3510000005d045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27509
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA920.3344360027627322No Hit
TACTGCAAGGAGGCTGTCTCTTATACA290.10542004434912211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT303.8006838E-421.00000218
GAGGCCG303.8006838E-421.00000210
GAGGTCG609.422365E-1021.00000210
GGAGAGC405.101081E-621.0000029
GGAGACG303.8006838E-421.0000029
GGAGAAG405.101081E-621.0000029
GAGAAGG303.8006838E-421.00000210
GAGGATG303.8006838E-421.00000210
GAGTGGG303.8006838E-421.00000210
TGCAAGG27100.021.0000024
AGAGGCG303.8006838E-421.00000211
GGATTTT558.061761E-921.00000218
TAGCTGT303.8006838E-421.00000218
TTCTGCA800.021.0000021
TCTGCAA800.021.0000022
GTCTTTT303.8006838E-421.00000218
GAGAGGG303.8006838E-421.00000210
CGGTTTT405.101081E-621.00000218
TCTTTTT558.061761E-921.00000219
GCATTTT303.8006838E-421.00000218
Produced by FastQC (version 0.11.2)