FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_44.3510000005d001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_44.3510000005d001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15004
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGCCACTCTGGGGGCTGTCTCTTAT210.1399626766195681No Hit
TTAGCCACTCTGAGGGCTGTCTCTTAT160.10663822980538523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA303.6898954E-421.0000021
CAGCCAC404.848509E-621.0000022
CTCTGGA800.021.0000028
TTGTTTT404.848509E-621.00000218
CTCTGCT608.421921E-1021.0000028
CTCTGCG850.021.0000028
CTCTGCA404.848509E-621.0000028
CTCTGAC850.021.0000028
AGCTTTT303.6898954E-421.00000218
TTTCTGT303.6898954E-421.00000221
CTGGGGT303.6898954E-421.00000210
TCTGGTG850.021.0000029
TCTGGTC303.6898954E-421.0000029
TCTGGGT303.6898954E-421.0000029
TCTGGGG1200.021.0000029
TCTGGGC608.421921E-1021.0000029
TCTGGCT303.6898954E-421.0000029
TCTGGCG608.421921E-1021.0000029
TCTGGAA303.6898954E-421.0000029
TTCTGTC404.848509E-621.00000221
Produced by FastQC (version 0.11.2)