FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_43.3510000005cfe9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_43.3510000005cfe9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16520
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA1600.9685230024213075No Hit
CTAGCTGGATGTCTGTCTCTTATACAC610.3692493946731235No Hit
CTAGCTGGATCTGTCTCTTATACACAT340.20581113801452786No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA270.16343825665859565No Hit
CTAGCTGGATGTCTGTCTCTTCTACAC270.16343825665859565No Hit
CTAGCTGGATGTGGGGCTGTCTCTTAT190.11501210653753026No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA190.11501210653753026No Hit
CTAGCTGGATGCTGTCTCTTCTACCCC170.10290556900726393No Hit
CTAGCTGGATGCTGTCTCTTCTACACA170.10290556900726393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTT3200.021.0000027
TGTGAGG404.898191E-621.00000210
GATGTTT651.0004442E-1021.0000028
GATGTTG1050.021.0000028
GATGTTA800.021.0000028
GATGTCT651.0004442E-1021.0000028
GATGTCG1050.021.0000028
GATGTCA800.021.0000028
CATTTTT651.0004442E-1021.00000219
ACTTTTT404.898191E-621.00000219
ATGTGGT651.0004442E-1021.0000029
ATGTGGA404.898191E-621.0000029
ATGTGCG651.0004442E-1021.0000029
GGCTTTT651.0004442E-1021.00000218
GATTTTT651.0004442E-1021.00000219
ATGTTTG404.898191E-621.0000029
GCTTTTT1050.021.00000219
AGCTGGA15150.021.0000023
TGTGTGC404.898191E-621.00000210
GGGCTTT404.898191E-621.00000217
Produced by FastQC (version 0.11.2)