FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_42.3510000005cfb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_42.3510000005cfb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12044
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG303.6300137E-421.09
ACTGTAC303.6300137E-421.09
ACTGAGT354.13508E-521.09
ACTGACG354.13508E-521.09
ACTGAAG303.6300137E-421.09
TTATTTT303.6300137E-421.018
TTCTGTC455.3744225E-721.021
CATTTTT404.713993E-621.019
TTTCAGA303.6300137E-421.01
TCCAGAG10150.021.02
GCCAGAG607.930794E-1021.02
CAGAGAC11550.021.04
TTCCAGA10150.021.01
TCAGAGA303.6300137E-421.03
TATTTTT701.0913936E-1121.019
GATTTTT354.13508E-521.019
GACTGTG751.8189894E-1221.08
GACTGTA800.021.08
AGAGACT11550.021.05
GACTGGT950.021.08
Produced by FastQC (version 0.11.2)