FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_40.3510000005cf7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_40.3510000005cf7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8226
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC630.7658643326039387No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC180.2188183807439825No Hit
GTTGAGCTTGACGGGGGGGGTTTTTTT150.18234865061998543No Hit
GTTGAGCTTGACAGCTGTCTCTTATAC100.12156576707999028No Hit
GTTGAGCTTGACGCTGTCTCTTATACA90.10940919037199125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT404.413934E-621.00000218
AATTTTT353.9294286E-521.00000219
GGATTTT404.413934E-621.00000218
GAGTTTT404.413934E-621.00000218
TTGACTG800.021.0000028
TTGACTA353.9294286E-521.0000028
TTGACGT709.094947E-1221.0000028
TTGACGA709.094947E-1221.0000028
TTGACCC353.9294286E-521.0000028
TTGACCA404.413934E-621.0000028
TTGACAA353.9294286E-521.0000028
CTTGACA1400.021.0000027
AGTTTTT709.094947E-1221.00000219
TGACGCG404.413934E-621.0000029
TGACGAG353.9294286E-521.0000029
CGTTTTT404.413934E-621.00000219
TTGTTTT303.4940854E-421.018
GTGTTTT250.003105648321.018
GTTGAGC6150.021.01
GGTGAGC454.949052E-721.01
Produced by FastQC (version 0.11.2)