FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_36.3510000005cf05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_36.3510000005cf05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18099
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA1000.5525167136305873No Hit
CAGAACGACAGTGGCTGTCTCTTATAC240.13260401127134097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGC455.7006946E-721.0000029
CAGTTGC303.7307912E-421.0000029
CAGTTCT303.7307912E-421.0000029
CAGTCAG303.7307912E-421.0000029
CAGCACG455.7006946E-721.0000021
GGCGTTT303.7307912E-421.00000217
CGCTTTT455.7006946E-721.00000218
AGTGGCG303.7307912E-421.00000210
AGTGGCA303.7307912E-421.00000210
AGTGGAG303.7307912E-421.00000210
CACGACA455.7006946E-721.0000024
ACCGACA608.785719E-1021.0000024
GTGGGGG303.7307912E-421.00000211
AGTGTGC303.7307912E-421.00000210
GCACGAC455.7006946E-721.0000023
GACCGAC608.785719E-1021.0000023
CAGACCG608.785719E-1021.0000021
AGCACGA455.7006946E-721.0000022
AGACCGA608.785719E-1021.0000022
ATCTGTC303.7307912E-421.00000220
Produced by FastQC (version 0.11.2)