FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_32.3510000005ce8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_32.3510000005ce8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13820
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC700.5065123010130246No Hit
GGATAGTTAGCCTGTCTCTTATACACA260.18813314037626627No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC140.10130246020260492No Hit
GGATAGTTAGCCGCTGTCTCTTATACA140.10130246020260492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT557.212293E-921.00000218
AATTTTT659.458745E-1121.00000219
GGATTTT404.80137E-621.00000218
TAGCCAG1100.021.0000028
TAGCCAA557.212293E-921.0000028
CCTTTTT404.80137E-621.00000219
GATTTTT950.021.00000219
CTAGTTA404.80137E-621.0000023
AGGTTTT557.212293E-921.00000218
GGGGTTT404.80137E-621.00000217
AGCCGTT404.80137E-621.0000029
AGCCGGC659.458745E-1121.0000029
TTAGTTA557.212293E-921.0000023
AGCCCGT303.668983E-421.09
AGCCCGG354.1944648E-521.09
AGCCTGG455.499496E-721.09
TTGTTTT303.668983E-421.018
AGCCCAG250.00321570121.09
GGGTTTT900.021.018
AGCCATT250.00321570121.09
Produced by FastQC (version 0.11.2)