FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_30.3510000005ce4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_30.3510000005ce4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC670.6973355537052456No Hit
AGTGTGACGAACCTGTCTCTTCTACAC270.2810158201498751No Hit
AGTGTGACGAACTGTCTCTTATACACA100.10407993338884262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGAA9300.021.0000025
TGGTTTT354.0231098E-521.018
TGTGACG8200.021.03
GGGTTTT354.0231098E-521.018
CAGTTTT303.5561965E-421.018
GGATTTT354.0231098E-521.018
GAACGCG354.0231098E-521.09
GAACGCC303.5561965E-421.09
GTTTTTT3150.021.020
GGTTTTT1150.021.019
GAGTTTT354.0231098E-521.018
TCTGTCT303.5561965E-421.021
AGTGTGA8150.021.01
GAACCGG303.5561965E-421.09
GAACTGG303.5561965E-421.09
CGAACTC303.5561965E-421.08
CCTTTTT303.5561965E-421.019
GGCTTTT354.0231098E-521.018
CGAACGT751.8189894E-1221.08
CGAACGC850.021.08
Produced by FastQC (version 0.11.2)