FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_28.3510000005cdf8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_28.3510000005cdf8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16516
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC1300.787115524340034No Hit
AGAGGACGAGCTGTCTCTTATACACAT580.35117461855170745No Hit
AGAGGACGAGATCTGTCTCTTCTACAC360.21797045289416325No Hit
AGAGGACGAGCTGTCTCTTATCCACAT200.1210946960523129No Hit
AGAGGACGAGATGCTGTCTCTTATACA170.10293049164446597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGG404.898191E-621.00000210
TGATTTT404.898191E-621.00000218
TGCTTTT404.898191E-621.00000218
GATCTGT404.898191E-621.00000210
AGATGGG1050.021.0000029
AGATGCA404.898191E-621.0000029
CGAGATG5750.021.0000027
CGGTTTT651.0004442E-1021.00000218
GCTTTTT1050.021.00000219
AGTTTTT1050.021.00000219
AGATTGG404.898191E-621.0000029
AGATTAT404.898191E-621.0000029
GAGATTC651.0004442E-1021.0000028
GAGATGT1300.021.0000028
GATGGGT250.00324247821.010
TTGTTTT354.2600164E-521.018
GGGTTTT900.021.018
CAGTTTT354.2600164E-521.018
TAGGACG303.711856E-421.02
AATTTTT608.62201E-1021.019
Produced by FastQC (version 0.11.2)