FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_26.3510000005cdb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_26.3510000005cdb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16846
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTGTCTCTTATACACATCTC1841.0922474177846373No Hit
ACTGTCTGTGTCCTGTCTCTTATACAC1660.9853971269144011No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC730.4333372907515137No Hit
ACTGTCTCTGTCTCTTATACCCATCTC240.14246705449364833No Hit
ACTGTCTGTGTCTGTCTCTTATACACA220.13059479995251097No Hit
ACTGTCTGTCTCTTATACACATCTCCG180.10685029087023626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT303.7157867E-421.00000218
GTGTCCC608.6583896E-1021.0000028
GTGTCAG950.021.0000028
GTGTCAA950.021.0000028
TGTCCTG303.7157867E-421.0000029
TGTCTAA303.7157867E-421.0000029
TGTTTTT1200.021.00000219
TGTCATT303.7157867E-421.0000029
TGTCATA303.7157867E-421.0000029
GTCTTTT303.7157867E-421.00000218
AGTTTTT1200.021.00000219
TGTGTCC2950.021.0000027
TGGTTTT404.9071095E-621.018
GTGTCTT800.021.08
GTGTCTC455.6515273E-721.08
GTGTCTA900.021.08
GTGTTTT455.6515273E-721.018
GGGTTTT800.021.018
GTGTCGC1300.021.08
GTGTCCG1250.021.08
Produced by FastQC (version 0.11.2)