FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_25.3510000005cd9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_25.3510000005cd9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC110.4398240703718513No Hit
CTTGGTTCGTTGTGGGGGGGTTTTTTT30.11995201919232307No Hit
CTTGGTTCGTTGGTATGGGGTTTTTTT30.11995201919232307No Hit
CTTGGTTCGTTGGGGGTCTGTTTTTTT30.11995201919232307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT652.0008883E-1121.0000027
TCGTTGC452.6357338E-721.0000027
CGTTGGA452.6357338E-721.0000028
TTCGTTG2500.021.06
GTTTTTT750.021.020
GGTTTTT352.674987E-521.019
GGTTCGT2500.021.04
CGTTGGG352.674987E-521.08
ATTTTTT800.021.020
GTTCGTT2500.021.05
TGGTTCG2250.020.9999983
AATTTTT250.002536513820.99999819
CTTGGTT2000.020.9999981
TATTTTT250.002536513820.99999819
TCGTTGG1000.020.9999987
TTGGTTC2000.020.9999982
CGTTGAG250.002536513820.9999988
TTTTTTT2200.020.52272621
CTTTTTT606.6029315E-919.25000220
TCGTTGA457.466355E-618.6666687
Produced by FastQC (version 0.11.2)