FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_23.3510000005cd64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_23.3510000005cd64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18098
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA950.5249198806497956No Hit
ATCTGCTATCCTGTCTCTTATACACAT320.1768151176925627No Hit
ATCTGCTATCCGGCTGTCTCTTATACA210.11603492098574428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT303.667506E-421.0527821
ATTTTTT4250.021.05277820
TTCTGTC250.003206899921.05277621
ATCTGTC250.003206899921.05277620
GTGTTTT701.0913936E-1120.99446118
GCCTTTT354.2977743E-520.99446118
AATTTTT1150.020.99446119
TGTCTGT303.737447E-420.99446119
TATGCTA455.715192E-720.9944612
TTATTTT303.737447E-420.99446118
CAATTTT303.737447E-420.99446118
TCCGGTC354.2977743E-520.9944619
TCCGGGT701.0913936E-1120.9944619
TCCGGGG1350.020.9944619
TCCGGGC455.715192E-720.9944619
GCTGCTA1250.020.9944612
TCCGGAA303.737447E-420.9944619
TATCCGT3950.020.9944617
TATCCCT303.737447E-420.9944617
GGCTTTT354.2977743E-520.99446118
Produced by FastQC (version 0.11.2)