FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_21.3510000005cd21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_21.3510000005cd21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19150
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC370.19321148825065274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT557.679773E-921.00000218
TTTGAAC557.679773E-921.0000023
ATCTTGA557.679773E-921.0000021
AATTTTT701.2732926E-1121.00000219
CTGGGTA354.306862E-521.0000029
CTGGGCG557.679773E-921.0000029
CTGGGAA354.306862E-521.0000029
TGTTTTT1400.021.00000219
CACTGTC354.306862E-521.00000219
CCTTGAA17600.021.0000022
ACTTTGA557.679773E-921.0000021
CTGGCGG354.306862E-521.0000029
CTGGCGA354.306862E-521.0000029
CTGGTGT354.306862E-521.0000029
CTGGTGG557.679773E-921.0000029
CTGGCCC354.306862E-521.0000029
TCTTGAA557.679773E-921.0000022
ACCTTGA17600.021.0000021
ACTGGTT1100.021.0000028
CTTTGAA557.679773E-921.0000022
Produced by FastQC (version 0.11.2)