FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_20.3510000005cd07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_20.3510000005cd07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23955
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT1890.7889793362554791No Hit
AGTCTCCTACGTCTGTCTCTTATACAC1490.6219995825506157No Hit
AGTCTCCTACCTGTCTCTTATCCACAT680.28386558129826756No Hit
AGTCTCCTACGTCTGTCTCTTCTACAC540.22542266750156542No Hit
AGTCTCCTACGCTGTCTCTTATACACA450.18785222291797118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCC557.930794E-921.0000021
GGGTTTT1100.021.00000218
GCCTCCT557.930794E-921.0000022
ACGTCTG557.930794E-921.0000029
ACGTCGG557.930794E-921.0000029
ACGTCGA303.780796E-421.0000029
ACGTTGG609.240466E-1021.0000029
TGATTTT303.780796E-421.00000218
CGCTTTT303.780796E-421.00000218
CGTGGGG609.240466E-1021.00000210
CCTCCTA557.930794E-921.0000023
GGGGGTT303.780796E-421.00000216
TACGTCT1200.021.0000028
TACGTCC1200.021.0000028
GAATTTT303.780796E-421.00000218
GCGTTTT609.240466E-1021.00000218
CTGTTTT303.780796E-421.00000218
ATATTTT557.930794E-921.00000218
ACGTGTG609.240466E-1021.0000029
ACGTGTC609.240466E-1021.0000029
Produced by FastQC (version 0.11.2)