FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_2.3510000005cab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_2.3510000005cab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10924
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA160.14646649578908824No Hit
GGTAGTGAGCTACGGCTGTCTCTTATA130.1190040278286342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACT556.7902874E-921.0000028
AGCTACC455.2792893E-721.0000028
AGCTAAG556.7902874E-921.0000028
GAGCTAT1800.021.0000027
CTAGGGG455.2792893E-721.00000210
GTGTTTT303.600078E-421.018
GGGTTTT709.094947E-1221.018
TAGTGAG8950.021.03
GCTAGTG303.600078E-421.09
ACGTTTT303.600078E-421.018
GCTAGGT404.647278E-621.09
GCTAGGG800.021.09
GCTAGGC404.647278E-621.09
GCTAGGA303.600078E-421.09
GAGTGAG607.6761353E-1021.03
GCTAGCG800.021.09
GCTAGCC505.991751E-821.09
GCTAGAG303.600078E-421.09
GCTAGAC250.003172502621.09
GTCGTGA505.991751E-821.02
Produced by FastQC (version 0.11.2)