FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_18.3510000005ccb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_18.3510000005ccb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9542
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA460.4820792286732341No Hit
GCATTGGATACCGGCTGTCTCTTATAC330.3458394466568854No Hit
GCATTGGATACCCTGTCTCTTATACAC300.3143994969608049No Hit
GCATTGGATACCGCCTGTCTCTTATAC230.24103961433661705No Hit
GCATTGGATACCCGCTGTCTCTTATAC190.19911968140850972No Hit
GCATTGGATACCGTCTGTCTCTTATAC180.1886396981764829No Hit
GCATTGGATACCGACTGTCTCTTATAC170.1781597149444561No Hit
GCATTGGATACCAGCTGTCTCTTATAC160.16767973171242925No Hit
GCATTGGATACCATCTGTCTCTTATAC140.1467197652483756No Hit
GCATTGGATACCGCTGTCTCTTATACA130.13623978201634876No Hit
GCATTGGATACCCTGTCTCTTCTACAC130.13623978201634876No Hit
GCATTGGATACCTGCTGTCTCTTATAC120.12575979878432195No Hit
GCATTGGATACCTACTGTCTCTTATAC110.1152798155522951No Hit
GCATTGGATACTGTCTCTTATACACAT100.10479983232026828No Hit
GCATTGGATACCTCCTGTCTCTTATAC100.10479983232026828No Hit
GCATTGGATACCCCTGTCTCTTATACA100.10479983232026828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA1600.021.0000027
TGTTTTT800.021.00000219
ATACCAT404.541631E-621.0000028
ATACCAA404.541631E-621.0000028
TGGTTTT250.003142455421.018
ACCGGTG303.552343E-421.010
ACCGGGG354.017284E-521.010
GTGTTTT455.1292955E-721.018
GGGTTTT250.003142455421.018
CATTGGA7950.021.02
GCCTTGG505.7838406E-821.01
GATACCT1900.021.07
GATACCG3600.021.07
GATACCC2250.021.07
TTTTTTT5600.021.021
ATTGGAT8400.021.03
GTTTTTT2600.021.020
GGTTTTT900.021.019
GTATTGG505.7838406E-821.01
GTCTGTC303.552343E-421.020
Produced by FastQC (version 0.11.2)