FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_17.3510000005cca9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_17.3510000005cca9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9740
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC550.5646817248459959No Hit
AACGTGCGTATGGCTGTCTCTTATACA320.32854209445585214No Hit
AACGTGCGTATGCTGTCTCTTCTACAC250.25667351129363447No Hit
AACGTGCGTATGCCTGTCTCTTATACA210.215605749486653No Hit
AACGTGCGTATCTGTCTCTTATACACA200.20533880903490762No Hit
AACGTGCGTATGGGCTGTCTCTTATAC180.18480492813141683No Hit
AACGTGCGTATGAGCTGTCTCTTATAC150.1540041067761807No Hit
AACGTGCGTATGGCCTGTCTCTTATAC130.13347022587268995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT658.185452E-1121.0000028
AACGTGC8850.021.0000021
GTATGCG658.185452E-1121.0000028
GGGTTTT404.5584566E-621.00000218
GTATGAG800.021.0000028
TATGACG404.5584566E-621.0000029
TCTGTCT404.5584566E-621.00000221
GATTTTT800.021.00000219
AGGTTTT404.5584566E-621.00000218
TATGGCA404.5584566E-621.0000029
TGGTTTT303.5599733E-421.018
GTATGTT354.028823E-521.08
GTATGTG505.8167643E-821.08
GTATGTC250.00314726521.08
GTATGTA303.5599733E-421.08
ATGGGGG354.028823E-521.010
GTATGGC1250.021.08
GTATGGA607.366907E-1021.08
GTATGCT505.8167643E-821.08
GTATGAT250.00314726521.08
Produced by FastQC (version 0.11.2)