FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_16.3510000005cc72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_16.3510000005cc72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC230.8963367108339828No Hit
GAACACCATAGCGGCTGTCTCTTATAC210.8183943881527669No Hit
GAACACCATAGCGCCTGTCTCTTATAC140.5455962587685114No Hit
GAACACCATAGCAGCTGTCTCTTATAC130.5066250974279034No Hit
GAACACCATAGCCTGTCTCTTCTACAC130.5066250974279034No Hit
GAACACCATAGCGTCTGTCTCTTATAC110.4286827747466875No Hit
GAACACCATAGCTGTCTCTTATACACA90.35074045206547155No Hit
GAACACCATAGCGACTGTCTCTTATAC80.3117692907248636No Hit
GAACACCATAGCATCTGTCTCTTATAC80.3117692907248636No Hit
GAACACCATAGCTCCTGTCTCTTATAC60.23382696804364772No Hit
GAACACCATAGCTGCTGTCTCTTATAC50.19485580670303976No Hit
GAACACCATAGCCCCTGTCTCTTATAC50.19485580670303976No Hit
GAACACCATAGCCCTGTCTCTTATACA40.1558846453624318No Hit
GAACACCATAGCCGCTGTCTCTTATAC40.1558846453624318No Hit
GAACACCATAGCGGCGGTGGTTTTTTT40.1558846453624318No Hit
GAACACCATAGCGGTTTAGGTTTTTTT40.1558846453624318No Hit
GAACACCATAGCGGTTGTTGTTTTTTT40.1558846453624318No Hit
GAACACCATAGCACCTGTCTCTTATAC30.11691348402182386No Hit
GAACACCATAGCGGGCGGGGTTTTTTT30.11691348402182386No Hit
GAACACCATAGCCGGGGGAGTTTTTTT30.11691348402182386No Hit
GAACACCATAGCTGTCTCTTCTACACA30.11691348402182386No Hit
GTACACCATAGCAGCTGTCTCTTATAC30.11691348402182386No Hit
GAACACCATAGCAACTGTCTCTTATAC30.11691348402182386No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGG302.6474395E-421.09
ACCATAG2500.021.05
TTTTTTT1500.021.021
GGTTTTT352.7053582E-521.019
ACACCAT2350.021.03
ATAGCGC302.6474395E-421.08
ATAGCAT302.6474395E-421.08
CACCATA2500.021.04
GATTTTT302.6474395E-421.019
CATAGCT402.719351E-621.07
CATAGCG1050.021.07
CATAGCC352.7053582E-521.07
CATAGCA750.021.07
CCATAGC2550.021.06
ATTTTTT701.8189894E-1221.020
CTTTTTT302.6474395E-421.020
GTTTTTT502.602792E-820.99999820
ATAGCGG452.6855378E-720.9999988
ATAGCAG250.002551460920.9999988
GAACACC2200.020.9999981
Produced by FastQC (version 0.11.2)