FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_14.3510000005cc3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_14.3510000005cc3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15136
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA1200.7928118393234672No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT320.21141649048625794No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC300.1982029598308668No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC210.13874207188160678No Hit
ATGCTCTGTTGCCCCTGTCTCTTATAC160.10570824524312897No Hit
ATGCTCTGTTGCGAGGCTGTCTCTTAT160.10570824524312897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTCT12500.021.0000021
TGGTTTT250.003229769421.018
TCCTTTT250.003229769421.018
TGTTGCG6900.021.07
TGTTGCC3050.021.07
GGGTTTT1150.021.018
TTGCAGA250.003229769421.09
AGCTCTG701.0913936E-1121.02
ACGCTCT506.401751E-821.01
AATTTTT608.440111E-1021.019
TGCGCGG404.852116E-621.010
ACGTTTT303.691492E-421.018
TGCGTGT303.691492E-421.010
GTCGTTT250.003229769421.017
CGCTCTG506.401751E-821.02
CGCTTTT250.003229769421.018
GTATTTT303.691492E-421.018
GGATTTT354.2288524E-521.018
TGCGAGG303.691492E-421.010
TAGTTTT250.003229769421.018
Produced by FastQC (version 0.11.2)