FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_13.3510000005cc15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_13.3510000005cc15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13819
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTCTGTCTCTTATACACA690.49931254070482667No Hit
GACTCTATGGTGGCTGTCTCTTATACA640.46313047253781026No Hit
GACTCTATGGTGTCTGTCTCTTATACA340.2460380635357117No Hit
GACTCTATGGTGCCTGTCTCTTATACA300.21709240900209859No Hit
GACTCTATGGTGACTGTCTCTTATACA290.20985599536869526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGATG404.80137E-621.0000029
GGGTTTT659.458745E-1121.00000218
CTATATG800.021.0000023
TATATGG800.021.0000024
AATTTTT800.021.00000219
ATGGTGG4400.021.0000027
ATGGTGC3200.021.0000027
GTGGGAG404.80137E-621.00000210
GTCTCTA800.021.0000021
CCTTTTT557.212293E-921.00000219
GATTCTA404.80137E-621.0000021
GATTTTT1100.021.00000219
ATATGGT800.021.0000025
TTCTATG404.80137E-621.0000023
GGTGGGA557.212293E-921.0000029
TCTCTAT800.021.0000022
ATTCTAT404.80137E-621.0000022
TGGTGTC800.021.0000028
AGATTTT404.80137E-621.00000218
TGGTGCG1100.021.0000028
Produced by FastQC (version 0.11.2)