FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_12.3510000005cbfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_12.3510000005cbfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19675
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC1270.6454891994917408No Hit
GAATCCTTACGCCTGTCTCTTCTACAC410.20838627700127066No Hit
GAATCCTTACGCTGTCTCTTATACACA240.12198221092757305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT651.03682396E-1021.00000218
GTGTTTT651.03682396E-1021.00000218
ACGCCTG455.7549005E-721.0000029
ACGCCGT404.9786995E-621.0000029
ACGCCGC651.03682396E-1021.0000029
ACGCTAG455.7549005E-721.0000029
CTCCTTA800.021.0000023
AATTTTT455.7549005E-721.00000219
ACGCACG404.9786995E-621.0000029
GCGGTTT404.9786995E-621.00000217
GGATTTT404.9786995E-621.00000218
ACTCCTT800.021.0000022
CATTTTT651.03682396E-1021.00000219
CCGTTTT455.7549005E-721.00000218
GAGTTTT455.7549005E-721.00000218
AACTTTT404.9786995E-621.00000218
TTCCTTA800.021.0000023
TATCCTT900.021.0000022
GGCTTTT404.9786995E-621.00000218
AGGTTTT404.9786995E-621.00000218
Produced by FastQC (version 0.11.2)