FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate3_10.3510000005cbc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate3_10.3510000005cbc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9672
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC1121.1579818031430935No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC420.4342431761786601No Hit
ACGTCTCTGCCTGTCTCTTATACACAT290.2998345740281224No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT160.1654259718775848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATTA404.554311E-621.0000028
TGCATAC303.5580932E-421.0000028
TGCATAA658.185452E-1121.0000028
ACGTCTC8250.021.0000021
TGTTTTT950.021.00000219
TCTGCAT9600.021.0000026
GCATGGT404.554311E-621.0000029
GCATGGG658.185452E-1121.0000029
GCATGGA404.554311E-621.0000029
CATGGGT303.5580932E-421.00000210
CTGTTTT404.554311E-621.00000218
CCCTTTT303.5580932E-421.00000218
AGATTTT303.5580932E-421.00000218
GCATCGG303.5580932E-421.0000029
GCATATG303.5580932E-421.0000029
CGTCTCT8250.021.0000022
TTGTTTT250.00314608121.018
CTCTGCA8700.021.05
TGCATTC250.00314608121.08
GGGTTTT505.808579E-821.018
Produced by FastQC (version 0.11.2)