FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_95.3510000005ca5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_95.3510000005ca5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86536
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC1150.1328926689470278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTG651.1823431E-1021.03881821
TTTTTTC303.8448774E-421.03881821
TTTTTCT250.003319129321.03881821
GTTTTCT354.4754674E-521.03881820
ATTTCTG1100.021.03881820
CTTCTGT354.4754674E-521.03881820
TGGTTTT1750.021.02663618
GTGTTTT1400.021.02663618
CAGTTTT601.0131771E-921.02663618
CCTATTT250.003330309621.02663617
GGCATTT558.569259E-921.02663617
GCGATTT250.003330309621.02663617
AATTCTG303.8599691E-421.02663619
ATGATTT250.003330309621.02663617
GTCGTTT354.4955283E-521.02663617
TAAGTTT250.003330309621.02663617
AATTTCT250.003330309621.02663619
GGTCTTT507.262315E-821.02663617
GAAGTTT303.8599691E-421.02663617
TGATCTG250.003330309621.02663618
Produced by FastQC (version 0.11.2)